In 1640, it became the cathedral of Tlaxcala due to the secularization imposed against the Franciscans.

Its facade is in baroque style and brilliantly combines the use of mortar, brick, and tile. It is presided over by the sculpture of San José.

Its single tower is located on the left side and consists of a single body with semicircular arches and fluted Tuscan pilasters on each side.

The interior is decorated in neoclassical style but retains interesting examples of baroque art in altars and retablos. On both sides of the sanctuary, there are beautiful golden retablos in the purest Churrigueresque style. The paintings in the sacristy, dating from the 18th century, are noteworthy.

Here, there are some relics that belonged to the Royal Chapel of the Indians: two stone carved holy water fonts, one with the image of Camaxtli, the pre-Hispanic god of the Tlaxcaltecas, and the other with the Spanish imperial shield.